Examined study of the impact of ethanol extract from Sirih China (Peperomia pellucida L.) ointment on the histological appearance of the epidermis and the duration of burn healing in mice (Mus musculus)

Armansyah Maulana Harahap, Salamah Salamah, Robiatun Rambe, Yurike Elanda, Nur Aini Khairunnisa, Indra Priawan

Abstract


Burns are a condition where there is damage to the epidermis layer down to the dermis layer. The skin structure sustains damage from the epithelium to the basal layer of the dermis. You can use the herbal Chinese pepper (Peperomia pellucida L.) for burn treatment. This study aims to evaluate the impact of administering Chinese betel leaf ointment, with variations in concentration and formula, on the duration of burn wound healing and the diameter of wound closure. Methods There were 30 Mus musculus mice in this study. Divided them into five groups: a regular group without burns, a positive control group that received gentamicin, treatment group 1 that received a low concentration 5% ethanol extract of Chinese betel leaf (EECB) ointment, a treatment group 2 that received a low concentration 10% EECB ointment, and a treatment group 3 that received a high concentration 15% EECB ointment. Results demonstrate wound closure activity by measuring the diameter of burn wounds treated with a 15% concentration of Chinese pepper ointment on the 18th day and a 15% concentration closed the burn wound. Histopathological differences were seen in the K3 group, where collagen density scored +3 (±0.06). We found a very weak positive correlation (0.000) between histopathology (collagen) and wound diameter and a strong negative correlation (-0.0527) between the histopathological parameter (collagen) and the duration of burn wound healing. Conclusion. The research concludes that the effectiveness of administering Chinese betel leaf ointment with a concentration of 15% was obtained

Keywords


Wound burn, Perperromia pellucida, Healing time, Diameter burn

Full Text:

PDF

References


Abdullah D.,2021, Penyembuhan Luka Sayat Pada Mencit Melalui Pemberian Gel Kefir, Penerbit Adab,Bandung

Adinda P., 2016. Pengaruh Pemberian Salep Ekstrak Daun Binahong (Anredera Cordifolia (Ten) Steenis) Terhadap Kepadatan Kolagen Tikus Putih (Rattus Norvegicus) Yang Mengalami Luka Bakar

Alemzadeh, E., & Oryan, A. (2018). Effectiveness Of A Crocus Sativus Extract On Burn Wounds In Rats. Planta Medica, 84(16), 1191–1200. Https://Doi.Org/10.1055/A-0631-3620

Aman B. Pulungan, S. D. (2014). Current Evidences In Pediatric Emergencies Management.

Athandau, D., R., Laut, M., Utami, T. (2022). Studi Literatur Uji Aktivitas Ekstrak Etanol Daun Anting-Anting (Acalypha indicaLinn.) Terhadap Penyembuhan Luka Bakar Pada Hewan Coba. Jurnal Veteriner Nusantara, Vol. VI No.30

Carolina,T.,Nughara, D., F., Fetriyah, D., H. (2022) Uji Aktivitas Ekstrak Kloroform Daun Sangkareho (Callicarpa longifolia lam.) Terhadap Penyembuhan Luka Sayat Pada Tikus Jantan Galur Wistar. Jurnal Surya Medika, Vol. 7 No. 2

Darotin, R., Nastiti, M., Ekaprasetia, F., (2023). Program Kelompok Pengenalan Kegawatdaruratan Dasar (Kopdar) Tentang Luka Bakar (Combustio) Di Smpn 12 Jember. J-Hics Journal Of Health Innovation And Community Service, 2(1), 38–43. Https://Jurnal.Stikesbanyuwangi.Ac.Id/Index.Php/Jhic/Index

Elmitra,2017, Dasar-Dasar Farmasetika Dan Sediaan Semi Solid, Deepblish, Yogyakarta.

Giri, M., D., 2021. Peran Metabolit Sekunder Tumbuhan dalam Pembentukan Kolagen pada Kulit Tikus yang Mengalami Luka Bakar. Jurnal Integrasi Obat Tradisional, Vol. 1 No. 1

Glinos, G. D., et al., 2017. Tahapan Penyembuhan luka. https://wasatchphotonics.com/applic ations/wound-healing-oct/ diakses 16 Maret 2023

Gomes, P. W. P et al. 2022. Chemical Composition of Leaves, Stem, and Roots of Peperomia pellucida (L.) Kunth. Journal molecules. Http://www.mdpi.com./journal/molecules

Hayah R., K., 2010. Pengaruh Pemberian Ekstrak Biji Jintan Hitam (Nigella sativa L.) Terhadap Kadar Asam Urat Darah Dan Gambaran Histologi Ginjal Mencit (Mus musculus) Hiperurisemia

Hiamawan, F.,(2022). Juru Rawat Descriptive Study Of First Aid For Mind Burn Management Of The Tegal City Orphanage In Fire Disaster (Vol. 2, Issue P). Juru Rawat : Jurnal Update Keperawatan Https://Ejournal.Poltekkes-Smg.Ac.Id/Ojs/Index.Php/Juk

Kalangi Bagaian, S. J. R., Fakultas, A.-H., Universitas, K., & Manado, S. R. (N.D.). Histofisiologi Kulit.

Karomah, S. (2019). Uji Ekstrak Tumbuhan Sirih Cina (Peperomia pellucida L.) Sebagai Antibakteri Terhadap Bakteri Staphylococcus Aureus Dan Staphy

Keputusan Menteri Kesehatan RI. 2015. Keputusan Menteri Kesehatan Republik Indonesia Nomor HK.02.02/Menkes/532/2015 tentang Formularium Nasional. Jakarta : Menteri Kesehatan RI.

Kim, Y., S., Choo,I.,H. Jeong, M.,J. 2012. Therapeutic Effect of Total Ginseng Saponin on Skin Wound Healing. Journal Ginseng Res Vol. 35, No. 3, 360-367

Majumder, P., Priya, AP., Satya V. 2011. Ethno-medicinal, Phytochemical and Pharmacological review of an amazing medicinal herb Peperomia pellucida (L.) HBK. Research Journal of Pharmaceutical, Biological and Chemical. Vol. 2, No. 358 – 364.

Mappa, Tiara., Edy, H. J., Kojong, Novel. 2013. Formulasi Gel Ekstrak Daun Sasaladahan (Peperomia pellucida (L.) H.B.K) dan Uji Efektivitasnya Terhadap Luka Bakar Pada Kelinci (Oryctolagus cuniculus). Jurnal Ilmiah Farmasi – Unsrat Vol. 2 No. 02

Miladiyah, I., Prabowo, B.R. 2012. Ethanolic extract of Anredera cordifolia (Ten.) Steenis leaves improved wound healing in guinea pigs, Universa Medicina. Vol. 31 (1): 4 - 11.

Mutiarawati, N., Puspitasari, S., Wati, S. H., Dwi, D., 2022. Keefektifan Sadusina (Salep Daun Sirih Cina) Sebagai Penyembuh Luka Bakar The Effectiveness Of Sadusina (Chinese Belt Leaf Ointment) As A Burns Healing. Jurnal Ilmiah Keperawatan (Scientific Journal Of Nursing), Vol 8, No 1

Novriansyah, R. 2008. Perbedaan Kepadatan Kolagen disekitar Luka Insısı Tikus Wistar yang Dibalut Kasa Konvensional dan Penutup Oklusif Hidrokoloid selama 2 dan 14 Han [Tesis] Program Pasca Sarjana Magister

Puspita, R., Harnis, Z. E., Harahap, N. I (2023). Uji Efektivitas Salep Ekstrak Etanol Daun Sirih Cina (Peperomia pellucida L.) Terhadap Penyembuhan Luka Eksisi Pada Tikus Putih (Rattus Norvegicus). In Jurnal Farmasi Dan Herbal (Vol. 5). Http://Ejournal.Delihusada.Ac.Id/Index.Php/Jpfh

Putri, F. M., Puspita, B. A., (2022) Uji Aktivitas Ektrak Etanol Daun Suruhan (Peperomia pellucida L.) Sebagai Penyembuhan Luka Bakar. Jurnal Inkofar. Vol.6 No.1 http://www.politeknikmeta.ac.id/meta/ojs

Rejeki, P.,S., Putri, E., Prasetya, R., 2018. Ovariektomi Pada Tikus Dan Mencit. Airlangga University Press. Surabaya

Rahimamullah,2022,Formulasi Dan Uji Efektivitas Salep Ekstrak Etanol Daun Talas (Colocasia Esculenta L.) Terhadap Penyembuhan Luka Bakar Derajat II Pada Kulit Tikus Putih Jantan Galur Wistar (Rattus Norvegicus)

Sianipar, A., Ginting, O., Gaho, G., 2024. Aktivitas Gel Ekstrak Daun Suruhan (Peperomia pellucida[L.] Kunth) Terhadap Penyembuhan Luka Bakar Pada Tikus Putih Jantan Galur Wistar. Jurnal Farmasi Kesehatan dan Sain. Vol. 2 No. 1

Suharto P, Etika AN. Ekstrak Jahe (Zingiber Officinale Roscoe) Berpengaruh Terhadap Kepadatan Serabut Kolagen Luka Insisi. Jurnal Ilmu Kesehatan Vol. 2019;8487(1):27–36.

Wigger-Alberti, et al. 2018. Penampakan penyembuhan kulit dari hari ke hari. https://www.elastoplast.com.au/first-aid/wound-care/moist-wound-healing. Diakses pada 16 Maret 2024

Wijayanti R., Syarifah M., Goenarwo E., 2015. Pengaruh Basis Salep Terhadap Sifat Fisik Sediaan Salep Ekstrak Kelopak Rosella (Hibiscus sabdariffa L.). Jurnal Media Farmasi Indonesia. Vol. 9 No.2

World Health Organization. 2018. Skin burn and prevalence by indication by WHO. WHO Global, accesed December 2024.

Yulianti. (2017). Penanganan Luka Bakar. Universitas Esa Unggul, 1-12.




DOI: https://doi.org/10.30743/best.v8i1.10460

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

 

https://scholar.google.co.id/citations?user=kvKzX3QAAAAJ&hl=id&authuser=4

Best Journal (Biology Education, Sains and Technology)

Program Studi Pendidikan Biologi, FKIP - Universitas Islam Sumatera Utara
Kampus Induk UISU Jl. Sisingamangaraja XII Teladan, Medan

Creative Commons License