Pemanfaatan Agrobacterium tumefaciens Pada Transgenesis Tumbuhan

Halifah Halifah


Abstract


Abstrak : Penelitian ini bertujuan untuk mengkaji pemanfaatan Agrobacterium tumefaciens dalam proses transgenesis tumbuhan. Metode yang digunakan adalah kajian literatur melalui pengumpulan, analisis, dan sintesis berbagai sumber ilmiah relevan guna menjawab pertanyaan penelitian. Hasil kajian menunjukkan bahwa A. tumefaciens merupakan bakteri gram negatif aerob obligat yang hidup secara alami di tanah serta dikenal sebagai penyebab penyakit crown gall pada berbagai tanaman. Dalam bidang bioteknologi, bakteri ini dimanfaatkan sebagai vektor transformasi genetik karena kemampuannya mentransfer DNA dari plasmid Ti ke dalam genom tanaman target. Transformasi yang diperantarai A. tumefaciens telah terbukti efektif pada berbagai tanaman dikotil maupun monokotil, dengan tingkat keberhasilan tinggi, kestabilan integrasi gen yang baik, serta biaya yang relatif lebih ekonomis dibandingkan metode transformasi lainnya

Keywords


Agrobacterium tumefaciens, transformasi genetik, tanaman transgenik.

Full Text:

PDF

References


Aggarwal, D., Datta, V., Tuli, H. S., Kumar, P., & Ramniwas, S. (2024). Agrobacterium tumefaciens-Mediated Genetic Transformation of Eclipta alba. International Journal of Plant Biology 2024, Vol. 15, Pages 641-651, 15(3), 641–651.

Azizi-Dargahlou, S., & pouresmaeil, M. (2024). Agrobacterium tumefaciens-Mediated Plant Transformation: A Review. Molecular biotechnology, 66(7), 1563–1580.

Bawa, A. S., & Anilakumar, K. R. (2012). Genetically modified foods: safety, risks and public concerns—a review. Journal of Food Science and Technology 2012 50:6, 50(6), 1035–1046.

Compton, M. E. (1994). Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell, Tissue and Organ Culture, 37(3), 217–242.

Gelvin, S. B. (2017). Integration of Agrobacterium T-DNA into the Plant Genome. Annual Review of Genetics, 51(Volume 51, 2017), 195–217. https://doi.org/10.1146/annurev-genet-120215-035320

Green, M. R., & Sambrook, J. (2017). Isolation of High-Molecular-Weight DNA Using Organic Solvents. Cold Spring Harbor Protocols, 2017(4), pdb.prot093450.

Hwang, H.-H., Yu, M., & Lai, E.-M. (2017). Agrobacterium -Mediated Plant Transformation: Biology and Applications . The Arabidopsis Book, 15(15), e0186.

Jeon, H., Bae, J., Hwang, S. H., Whang, K. Y., Lee, H. S., Kim, H., & Kim, M. S. (2019). MRPrimerW2: An enhanced tool for rapid design of valid high-quality primers with multiple search modes for qPCR experiments. Nucleic Acids Research, 47(W1), W614–W622. https://doi.org/10.1093/nar/gkz323

Lee, J. A., Baugh, A. C., Shevalier, N. J., Strand, B., Stolyar, S., & Marx, C. J. (2021). Cross-feeding of a toxic metabolite in a synthetic lignocellulose-degrading microbial community. Microorganisms, 9(2), 1–20.

Lorenz, T. C. (2012). Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies. Journal of Visualized Experiments : JoVE, 63, 3998.

MINARSIH, H., SUBIYARTI, D., RIYADI, I., PUTRA, S. M., & AMBARSARI, L. (2015). Evaluasi varietas, sumber eksplan dan strain Agrobacterium terhadap keberhasilan transformasi tebu dengan gen P5CS Evaluation of varieties, explant sources, and Agrobacterium strains for successful sugarcane transformation using P5CS gene. Menara Perkebunan, 83(1).

Møller, T. A., Booth, T. J., Shaw, S., Møller, V. K., Frandsen, R. J. N., & Weber, T. (2025). ActinoMation: A literate programming approach for medium-throughput robotic conjugation of Streptomyces spp. Synthetic and Systems Biotechnology, 10(2), 667–676.

Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D., & Kamoun, S. (2017). Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Scientific Reports, 7(1), 1–6.

Phadtare, S., & Severinov, K. (2010). RNA remodeling and gene regulation by cold shock proteins. RNA Biology, 7(6), 788–795.

Qaim, M. (2020). Role of New Plant Breeding Technologies for Food Security and Sustainable Agricultural Development. Applied Economic Perspectives and Policy, 42(2), 129–150.

Rotinsulu, S., Fatimawali, F., & Tallei, T. E. (2019). TRANSFORMASI PLASMID YANG MENGANDUNG GEN merB PADA BAKTERI Escherichia coli TOP-10. PHARMACON, 8(2), 290–297. https://doi.org/10.35799/pha.8.2019.29294

Snyder, H. (2019). Literature review as a research methodology: An overview and guidelines. Journal of Business Research, 104, 333–339.

Tranfield, D., Denyer, D., & Smart, P. (2003). Towards a Methodology for Developing Evidence-Informed Management Knowledge by Means of Systematic Review. British Journal of Management, 14(3), 207–222. https://doi.org/10.1111/1467-8551.00375

Wacogne, B., Belinger Podevin, M., Vaccari, N., Koubevi, C., Codjiová, C., Gutierrez, E., Davoine, L., Robert-Nicoud, M., Rouleau, A., & Frelet-Barrand, A. (2024). Concentration vs. Optical Density of ESKAPEE Bacteria: A Method to Determine the Optimum Measurement Wavelength. Sensors, 24(24). 60

Wahyuni, F. D., Sidiq, M. A., Seprianto, S., & Saraswati, H. (2022). TRANSFORMASI PLASMID REKOMBINAN pRI_101-AN MEMBAWA SISIPAN GEN cryIII MELALUI Agrobacterium tumefaciens. BERITA BIOLOGI, 21(1), 71–78




DOI: https://doi.org/10.30743/best.v8i2.13095

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.