Penentuan Kadar Vitamin D2 Pada Ekstrak Etanol 96% Jamur Sawit (Volvariella Volvacea) Dengan Metode High Performance Liquid Chromatography (HPLC)

Assyifa Ainalaysia, Oky Hermansyah, Maria Eka Patri Yulianti, Putri Mulia, Rose Intan Perma Sari, Sutra Elvyanti


Abstract


Vitamin D plays a crucial role in maintaining bone and dental health and enhancing the body’s immune system function. Vitamin D deficiency has spurred the exploration of alternative sources of vitamin D from natural food sources that may contain vitamin D₂. The palm mushroom (Volvariella volvacea) is one such natural food source known to contain vitamin D₂. This study aims to determine the vitamin D₂ content in a 96% ethanol extract of the palm mushroom (Volvariella volvacea). The vitamin D₂ content was determined using High Performance Liquid Chromatography (HPLC) with a BPFI standard vitamin D₂ reference standard. The results showed that the yield of the 96% ethanol extract of the palm mushroom was 10%. The maximum wavelength of the vitamin D₂ standard reference was obtained at 265 nm with an optimized mobile phase of Pro HPLC acetonitrile : Pro HPL methanol (90:10) and a flow rate of 1 mL/minute. The linear equation of the vitamin D2 calibration curve is y = 669.36x + 654.54 with a correlation coefficient (r) of 0.997, indicating good linearity of the analytical method. From this study, it can be concluded that palm mushrooms have potential as a source of vitamin D2.

Keywords


96% ethanol, palm fungus, High-Performance Liquid Chromatography, Vitamin D2

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DOI: https://doi.org/10.30743/best.v9i1.13345

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