Efek Pericarpium Manggis (Garcinia mangostana L.) Terhadap Protein Spermatozoa Epididimal Mencit Setelah Dipapar 2-Methoxyethanol

Putri Ayu Ika Setiyowati

Abstract


Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak fraksi pericarpium manggis (Garcinia mangostana L.) dengan variasi kepolaran dan dosis terhadap profil protein epididimal spermatozoa mencit (Mus musculus) yang terpapar 2-Methoxyethanol. Penelitian ini menggunakan 50 ekor mencit jantan strain BALB/C dengan berat badan 25-30 gram yang dibagi menjadi 8 kelompok; kelompok kontrol positif diberi 0,05 ml larutan CMC 0,05 % selama 40 hari , kelompok kontrol negatif diberi 200 mg/kg BB 2-Methoxyethanol selama 35 hari, kelompok perlakuan (P1, P2, P3, P4, P5, dan P6) diberi 200 mg/kg BB 2-Methoxyethanol selama 5 hari, dilanjutkan dengan pemberian ekstrak fraksi dan dosis, kelompok P1 dan P2 diberi ekstrak fraksi non polar pericarpium manggis dengan dosis 0,6 mg/kg BB dan 3 mg/kg BB selama 35 hari , P3 dan P4 diberi ekstrak fraksi semi polar dengan dosis 4 mg/kg BB dan 20 mg/kg BB selama 35 hari, P5 dan P6  diberi ekstrak fraksi polar pericarpium manggis dengan dosis 0,4 mg/kg BB dan 2 mg/kg BB selama 35 hari. Protein diisolasi dari spermatozoa yang dikoleksi dari epididimis, kemudian diukur konsentrasinya dan dianalisis menggunakan elektroforesis SDS-PAGE. Data yang diperoleh dianalisis secara deskriptif dengan membandingkan profil protein kelompok kontrol dan kelompok perlakuan. Hasil penelitian menunjukkan bahwa ekstrak fraksi polar pericarpium manggis dengan dosis 0,4 mg/kg BB dapat berpengaruh dalam memulihkan profil protein (20 kDa) dari spermatozoa epididimal mencit setelah terpapar 2-Methoxyethanol

Keywords


Garcinia mangostana L., protein spermatozoa, spermatozoa epididimal, 2-Methoxyethanol.

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References


Agarwal, A., & Prabakaran, S. A. (2005). Mechanism, measurement and prevention of O.S. Indian Journal of Experimental Biology, 43(November), 963–974.

Arrotéia, K. F., Garcia, P. V., & Barbieri, M. F. (2011). The Epididymis : Embryology , Structure , Function and Its Role in Fertilization and Infertility.

Awda, B. J., Mackenzie-Bell, M., & Buhr, M. M. (2009). Reactive Oxygen Species and Boar Sperm Function1. Biology of Reproduction, 81(3), 553–561. https://doi.org/10.1095/biolreprod.109.076471

El-Shahat, K. H., Taysser, M. I., Badr, M. R., & Zaki, K. (2016). Effect of L-arginine treatment on motility, hyperactivity, acrosome reaction of ejaculated ram spermatozoa. Animal Reproduction, 13(2), 75–80. https://doi.org/10.21451/1984-3143-AR788

Fàbrega, A., Guyonnet, B., Dacheux, J. L., Gatti, J. L., Puigmulé, M., Bonet, S., & Pinart, E. (2011). Expression, immunolocalization and processing of fertilins ADAM-1 and ADAM-2 in the boar (sus domesticus) spermatozoa during epididymal maturation. Reproductive Biology and Endocrinology, 9, 1–13. https://doi.org/10.1186/1477-7827-9-96

Fitri, R. A., Sumarmin, R., & Yuniarti, E. (2017). BioScience|Volume 1 no. 1(2). https://doi.org/10.24036/bsc.v1i2.7718

Gaudreault, C., El Alfy, M., Légaré, C., & Sullivan, R. (2001). Expression of the Hamster Sperm Protein P26h During Spermatogenesis1. Biology of Reproduction, 65(1), 79–86. https://doi.org/10.1095/biolreprod65.1.79

Han, C., Park, I., Lee, B., Jin, S., Choi, H., Kwon, J. T., Kwon, Y. il, Kim, D. H., Park, Z. Y., & Cho, C. (2011). Identification of heat shock protein 5, calnexin and integral membrane protein 2B as Adam7-interacting membrane proteins in mouse sperm. Journal of Cellular Physiology, 226(5), 1186–1195. https://doi.org/10.1002/jcp.22444

Hidayat, E., Susanti, R., & Marianti, A. (2016). Protein Profile and Mda Spermatozoa Levels of Hyperglycemic Mice Fed By Bean Sprouts Extract. Indonesian Journal of Pharmacy, 26(4), 192. https://doi.org/10.14499/indonesianjpharm26iss4pp192

Kim, E., Lee, J. W., Baek, D. C., Lee, S. R., Kim, M. S., Kim, S. H., Kim, C. S., Ryoo, Z. Y., Kang, H. S., & Chang, K. T. (2010). Processing and subcellular localization of ADAM2 in the Macaca fascicularis testis and sperm. Animal Reproduction Science, 117(1–2), 155–159. https://doi.org/10.1016/j.anireprosci.2009.04.002

Kurniawati, M., Mahdi, C., & Aulanni’am, A. (2014). The Effect Of Juice Mangosteen Rind (Garcinia Mangostana L.) To Blood Sugar Levels And Histological Of Pancreatic Rats With The Induction Of Streptozotocin. The Journal of Pure and Applied Chemistry Research, 3(1), 1–6. https://doi.org/10.21776/ub.jpacr.2014.003.01.145

Lea, I. A., Widgren, E. E., & O’Rand, M. G. (2004). Association of sperm protein 17 with A-kinase anchoring protein 3 flagella. Reproductive Biology and Endocrinology, 2, 1–7. https://doi.org/10.1186/1477-7827-2-57

Mahasri, G., Fajriah, U., & SUBEKTI, S. (2010). Karakterisasi protein Lernaea cyprinacea dengan Metode Elektroforesis SDS-PAGE. Jurnal Ilmiah Perikanan Dan Kelautan, 2(1), 61–67.

Moongkarndi, P., Kosem, N., Kaslungka, S., Luanratana, O., Pongpan, N., & Neungton, N. (2004). Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. Journal of Ethnopharmacology, 90(1), 161–166. https://doi.org/10.1016/j.jep.2003.09.048

Nagdas, S. K., McLean, E. L., Richardson, L. P., & Raychoudhury, S. (2014). Identification and characterization of TEX101 in bovine epididymal spermatozoa. Biochemistry Research International, 2014. https://doi.org/10.1155/2014/573293

Rahmaningtyas, E., Yusa, N. M., & Puspawati2, N. N. (2013). Pengaruh penambahan cmc (. Teknologi Pertanian Universitas Udayana, 34(1), 1–7. https://ojs.unud.ac.id/index.php/itepa/article/download/27494/17403/.

Starek-͆wiechowicz, B., Szymczak, W., Budziszewska, B., & Starek, A. (2015). Testicular effect of a mixture of 2-methoxyethanol and 2-ethoxyethanol in rats. Pharmacological Reports, 67(2), 289–293. https://doi.org/10.1016/j.pharep.2014.09.011

Weecharangsan, W., Opanasopit, P., Sukma, M., Ngawhirunpat, T., Sotanaphun, U., & Siripong, P. (2006). Antioxidative and neuroprotective activities of extracts from the fruit hull of mangosteen (Garcinia mangostana Linn.). Medical Principles and Practice, 15(4), 281–287. https://doi.org/10.1159/000092991




DOI: https://doi.org/10.30743/best.v3i2.2809

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