Antibacterial Potential Ethanol Extract of Papaya Leaves (Carica papaya Linn.) Towards Salmonella typhi

Mahatir Muhammad, Nasri Nasri, Vera Estefania Kaban, Denny Satria, Henni Cintya

Abstract


Infectious diseases are a problem of great concern throughout the world. Bacterial infections such as Salmonella typhi often attack children and cause diarrhea and typhoid fever. Salmonella typhi is a beflagellated Gram negative bacteria that can be transmitted through contaminated food, drink, or water. Treatment of infections using antibiotics, where often the use of antibiotics is not achieved on the therapeutic target due to lack of patient compliance in taking antibiotics, will cause new problems, namely problems related to antibiotic resistance. Papaya leaf is a plant originating from the Caricaceae family which, based on studies, has been reported to have antimicrobial, antidiabetic, and immunomodulatory effects. The purpose of this study was to examine the antibacterial potential of ethanol extract of papaya leaves (Carica papaya Linn.) against Salmonella typhi bacteria. This research method uses the agar diffusion method (Kirby-Bauer) with various concentrations to determine the minimum inhibitory concentration (MIC) and the streaking method to determine the minimum killing concentration (MBC). The minimum inhibitory concentration was obtained at a concentration of 3.125 mg/mL with an inhibitory zone diameter of 6.70 ± 0.20 mm and a minimum killing concentration of 50 mg/mL. The index activity value at a concentration of 300 mg/mL was 0.688 ± 0.05. So it can be concluded that the ethanol extract of papaya leaves has potential antibacterial activity against Salmonella typhi bacteria.

Keywords


Carica papaya Linn., Antibacterial, MIC, MBC, Salmonella typhi

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References


Adedeji, W. A. (2016). THE TREASURE CALLED ANTIBIOTICS. Annals of Ibadan Postgraduate Medicine, 14(2), 56–57.

Ayoola, P. B., & Adeyeye, A. (2010). Phytochemical and nutrient evaluation of Carica papaya (pawpaw) leaves. Ijrras, 5(3), 325–328.

Dewi, M. A., Ratnawati, J., & Sukmanengsih, F. (2015). Aktivitas Antimikroba Ekstrak Etanol Dan Fraksi Pelepah Aren (Arenga Pinnata Merr) Terhadap Propionibacterium Acnes dan Staphylococcus Aureus. Kartika: Jurnal Ilmiah Farmasi, 3(1), 43–48.

Dwicahyani, T., Sumardianto, S., & Rianingsih, L. (2018). UJI BIOAKTIVITAS EKSTRAK TERIPANG KELING Holothuria atra SEBAGAI ANTIBAKTERI Staphylococcus aureus dan Escherichia coli. Jurnal Pengolahan Dan Bioteknologi Hasil Perikanan, 7(1), 15–24.

Firdaus, T. (2014). Efektivitas ekstrak bawang dayak (eleutherine palmifolia) dalam menghambat pertumbuhan bakteri staphylococcus aureus.

Fitriana, F. (2010). Pengaruh Metode Ekstraksi Terhadap Aktivitas Antimikroba Ekstrak Metanol Daun Jati (Tectona grandis LF) [PhD Thesis]. Universitas Islam Negeri Alauddin Makassar.

Handayani, K., Putri, A. E., & Martha, R. D. (2020). UJI AKTIVITAS ANTIBAKTERI FRAKSI BATANG PEPAYA (Carica Papaya Linn.) TERHADAP BAKTERI Staphylococcus Aureus. JOPS (Journal Of Pharmacy and Science), 4(1), 21–30.

Harahap, U., Dalimunthe, A., Hertiani, T., Muhammad, M., Nasri, & Satria, D. (2021). Antioxidant and antibacterial activities of ethanol extract of Vernonia amygdalina Delile. Leaves. AIP Conference Proceedings, 2342(1), 080011.

Haro, G., Iksen, I., & Nasri, N. (2020). Identification, characterization and antibacterial potential of probiotic lactic acid bacteria isolated from naniura (A traditional batak fermented food from carp) against Salmonella typhi. Rasayan Journal of Chemistry, 13(1), 464–468.

Hati, A. K., Dyahariesti, N., & Yuswantina, R. (2019). Penetapan Kadar Flavonoid Dan Uji Aktivitas Antibakteri Ekstrak Sereh (Cymbopogon nardus) Dan Temu Kunci (Boesnbergia pandurata Roxb) Terhadap Bakteri Streptococcus Mutans. Indonesian Journal of Pharmacy and Natural Product, 2(2).

Kuspradini, H., Putri, A. S., Egra, S., & YANTI, Y. (2019). In vitro antibacterial activity of essential oils from twelve aromatic plants from East Kalimantan, Indonesia. Biodiversitas Journal of Biological Diversity, 20(7).

Mahdiva, A. S., Febriani, H., & Rahmadina. (2021). UJI AKTIVITAS ANTIBAKTERI GETAH JARAK PAGAR (Jatropha curcas L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus epidermidis. BEST Journal (Biology Education, Sains and Technology), 4(2), 109–114. https://doi.org/10.30743/best.v4i2.4413

Mathur, R., Oh, H., Zhang, D., Park, S.-G., Seo, J., Koblansky, A., Hayden, M. S., & Ghosh, S. (2012). A Mouse Model of Salmonella Typhi Infection. Cell, 151(3), 590–602. https://doi.org/10.1016/j.cell.2012.08.042

Nasri, N., Kaban, V. E., Gurning, K., Syahputra, H. D., & Satria, D. (2022). Aktivitas Antibakteri Ekstrak Etanol Daun Pepaya (Carica papaya Linn.) Terhadap Bakteri Pseudomonas aeruginosa. INSOLOGI: Jurnal Sains Dan Teknologi, 1(3), 252–259. https://doi.org/10.55123/insologi.v1i3.438

Nasri, N., Kaban, V. E., Syahputra, H. D., & Satria, D. (2022). Aktivitas Antibakteri Ekstrak Etanol Daun Alpukat (Persea americana Mill) Terhadap Escherichia coli, Salmonella typhi, dan Pseudomonas aeruginosa. Herbal Medicine Journal, 5(1), 13–19.

Retnaningsih, A., Primadiamanti, A., & Febrianti, A. (2019). UJI DAYA HAMBAT EKSTRAK ETANOL DAUN UNGU (Graptophyllum pictum (L.) GRIFF) TERHADAP BAKTERI Staphylococcus epidermidis DAN BAKTERI Propionibacterium acnesPENYEBAB JERAWAT DENGAN METODE CAKRAM. Jurnal Analis Farmasi, 4(1).

Singh, S. P., Kumar, S., Mathan, S. V., Tomar, M. S., Singh, R. K., Verma, P. K., Kumar, A., Kumar, S., Singh, R. P., & Acharya, A. (2020). Therapeutic application of Carica papaya leaf extract in the management of human diseases. DARU Journal of Pharmaceutical Sciences, 28(2), 735–744. https://doi.org/10.1007/s40199-020-00348-7.




DOI: https://doi.org/10.30743/best.v5i2.5717

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Program Studi Pendidikan Biologi, FKIP - Universitas Islam Sumatera Utara
Kampus Induk UISU Jl. Sisingamangaraja XII Teladan, Medan

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