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DNA BARCODING OF HARIMONTING (RHODOMYRTUS TOMENTOSA) USING THE TRNL-F GENE
Abstract
DNA Barcoding is one or more short gene sequences taken from a standard part of the genome and used to identify species. Harimonting plant is a wild plant, but in some places this plant is used as an ornamental plant. This study aims to determine the molecular characteristics of the trnL-F gene in Rhodomyrtus tomentosa, to determine the genetic diversity of Rhodomyrtus tomentosa using the trnL-F gene, and to examine the phylogenetic relationships of Rhodomyrtus tomentosa using the trnL-F gene. This research method was carried out in stages: sample collection, DNA isolation, PCR (Polymerase Chain Reaction), electrophoresis, sequencing and analysis of sequence results. Morphological characterization of Rhodomyrtus tomentosa plants collected from Tara Bunga Hill was seen from the results of DNA barcodes and observations of visible morphological characters and literature studies. The results of the Rhodomyrtus tomentosa DNA barcode from the results of molecular analysis of Rhodomyrtus tomentosa obtained the results of a sequence of 359 bp. The plant sequences of Rhodomyrtus tomentosa were analyzed using the MEGA 11 application. Genetic distance calculation (pairwise distance), GC content calculation, and nucleotide diversity were performed. Calculation of GC content showed that the value of GC content in Rhodomyrtus tomentosa was lower than AT content which indicated that Rhodomyrtus tomentosa species were more primitive. Based on the phylogenetic tree reconstruction, all species are divided into 2 clades.
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Adjie, B., Takamiya, M., Ohta, M., Oshawa, T. A., & Watano, Y. (2008). Molecular Phylogeny of the Lady Fern Genus Athyrium in Japan Based on Chloroplast rbcL and trnl-trnF Sequences.
Chen, C. W., Huang, Y. M., Kuo, L. Y., Nguyen, Q. D., Luu, H. T., Callado, J. R., Farrar, D. R., & Chiou, W. L. (2013). TrnL-F is a powerful marker for DNA identification of field vittarioid gametophytes (Pteridaceae). Journal Annals of Botany, 111(4), 663–673.
Chen, S., Yao, H., Han, J., Liu, C., Song, J., Shi, L., Zhu, Y., Ma, X., Gao, T., Pang, X., Luo, K., Li, Y., Li, X., Jia, X., Lin, Y., & Leon, C. (2010). Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. Journal PLoS ONE, 5(1).
de Groot, G. A., During, H. J., Maas, J. W., Schneider, H., Vogel, J. C., & Erkens, R. H. J. (2011). Use of rbcL and trnL-F as a two-locus DNA barcode for identification of NW-European ferns: An ecological perspective. Jou.rnal PLoS ONE, 6(1).
Hamid, H. A., Roziasyahira Mutazah, S. S. Z., & Yusoff, M. M. (2017). Rhodomyrtus tomentosa: A phytochemical and pharmacological review. Asian Journal of Pharmaceutical and Clinical Research 10 (1): 10–16. Innovare Academics Sciences Pvt. Ltd..
Hanifa, R, Y,. Sri P,. Rejeki, S, F,. & Hermin, P. (2021). Identifikasi Molekuler Jeruk Nipis Tegal Berdasarkan Fragmen Gen 18s Ribosomal RNA. Jurnal Bioteknologi & Biosains Indonesia, 8(2): 244-254.
Hao, D. C., Huang, B. L., Chen, S. L., & Mu, J. (2009). Evolution of the Chloroplast trnL-trnF region in the gymnosperm lineages taxaceae and cephalotaxaceae. Biochemical Genetics, 47(5–6): 351–369.
Hapsari, L. (2015). Keragaman dan Kekerabatan Genetik Pisang (Musa L.) di Jawa Timur berdasarkan Sekuen Daerah Internal Transcribed Spacer. UniversitasBrawijaya. Malang.
Hebert, P. D. N., Cywinska, A., Ball, S. L., & DeWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270 (1512): 313–321.
Hoggard, G. D., Kores, P. J., Molvray, M., & Hoggard, R. K. (2004). The Phylogeny Of Gaura (Onagraceae) Based On ITS, ETS, And TRNL-F Sequence Data 1. In American Journal of Botany, 91 (1): 139-148.
Kress, W. J., & Erickson, D. L. (2007). A Two-Locus Global DNA Barcode for Land Plants: The Coding rbcL Gene Complements the Non-Coding trnH-psbA Spacer Region. Journal PLoS ONE, 2 (6): 1-10
Ramadany, Z. (2020). Desain Primer Internal Transcribed Spacer (ITS) Sebagai Dasar Identifikasi Molekuler Anggrek Obat Dendrobium discolor Lindl. Skripsi.
Techen, N., Parveen, I., Pan, Z., & Khan, I. A. (2014). DNA barcoding of medicinal plant material for identification. In Current Opinion in Biotechnology, 25 (1): 103–110. Elsevier Ltd.
Wang, D.-Y., Wang, Q., Wang, Y.-L., Xiang, X.-G., Huang, L.-Q., & Jin, X.-H. (2017). Evaluation of DNA barcodes in Codonopsis (Campanulaceae) and in some large angiosperm plant genera. Journal PLos One: 1-14.
Yenihayati. (2018).Identification of secondary metabolites. Jurnal Pendidikan Teknologi Dan Kejuruan BALANGA, 6(1): 1-12.
Yuliana, I., Asnawati, & Maria Ulfa. (2021). Potensi Daun Karamunting Sebagai Fitofarmaka Penyakit Diabetes Mellitus. Lambung Mangkurat University Press. Banjarmasin.
Zhang, D., Duan, L., & Zhou, N. (2014). Application of DNA barcoding in Roscoea (Zingiberaceae) and a primary discussion on taxonomic status of Roscoea cautleoides var. pubescens. Journal Biochemical Systematics and Ecology, 52: 14–19.
DOI: https://doi.org/10.30743/best.v7i1.8440
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